gene targeting

Gene targeting is a rising technology in molecular biology, which is defined as the introduction of exogeneous DNA to specific site in genome by homologous recombination, and consequently change the hereditary character of the cell.

基因打靶技术是一项新兴的分子生物学技术，是利用外源DNA与受体细胞染色体DNA上的同源序列之间发生重组，并整合在预定位点上，从而改变细胞遗传特性的方法。

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It led to a new technology -- gene targeting.

并诞生了一门新技术 ― ―基因靶向.

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The Present Research of Gene Targeting in Plants

植物基因打靶研究现状

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Construction of the TYR gene targeting vector ( 1) Cloning of the targeting homology arm sequences of the TYR gene.

构建TYR基因打靶载体（1）克隆TYR基因的打靶同源臂序列。

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It led to a new technology& gene targeting.

并诞生了一门新技术――基因靶向。

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Transposon mutagenesis and suicide vectors are mainly used for gene targeting in other microbes.

在其他微生物中主要应用转座子突变和自杀载体进行基因打靶。

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Gene targeting technology and its application in research of animal model of human disease were briefed in this paper.

本文就基因打靶技术及其在人类疾病动物模型研究中的应用做一介绍。

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Gene Targeting and Its Affecting Factors

基因打靶技术及其影响因素

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Application and Progress of Filamentous Fungi Gene Targeting

丝状真菌基因敲除技术研究进展

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DNA repair approach has focused on gene targeting or gene replacement whereby the defective gene is removed and a normal gene is inserted at the same position in the chromosome and so it maintains the corrected gene's expression in the natural context.

基因原位修复策略是一种直接的基因疗法，它针对基因缺陷部位进行修复，保持了基因原有的染色质环境，因此是一种理想的靶向性基因治疗方式。

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Gene targeting is a useful protocol to modify the cell genome based on the homologous recombination.

基因敲除技术是建立在同源重组（Homologous Recombination，HR）基础之上对基因组进行遗传修饰的一种较理想的方法。

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Construction and application of gene targeting replacement vector

基因打靶置换型载体的构建和应用研究

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Construction of chicken ovalbumin gene targeting vector containing the Cre recombinase recognition site

基于Cre重组酶体系的鸡卵清蛋白基因打靶载体的构建

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Gene Targeting in Plants

植物的基因打靶

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MMP-9 Gene Targeting Therapy and Mechanism Study of Herpes Simplex Encephalitis

单纯疱疹病毒性脑炎发病机制及靶向MMP-9基因治疗的研究

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The technique of multiple locus gene targeting on repetitive sequences will resolve a part of these problems.

以重复序列为靶位点的多位点基因打靶技术，将部分解决目前存在的打靶效率低、安全性差等问题。

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A Preliminary Study on the Human TPO Gene Targeting Vector Construction and Bovine Somatic Cell Transfection

人TPO基因牛体细胞定点整合载体构建与细胞转染研究

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Construction of Human Pol ι Somatic Gene Targeting Vector

人DNA聚合酶Polι体细胞基因敲除载体的构建

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Gene targeting technology, has brought the possibility of human transformation of life.

基因靶向技术的进步，带来了人类改造生命的可能。

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Studies on the Application of Gene Targeting

基因打靶技术的应用研究

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Cloning of the rRNA Genes and Construction of the Multiple Locus Gene Targeting Vector of Bos Taurus

奶牛rRNA基因的克隆及多位点基因打靶载体的构建

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Expression of Goat β-casein Gene Targeting Vector in Mammary Gland Cell

山羊β-casein位点打靶载体在乳腺上皮细胞中的表达研究

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Construction of a Mouse Novel Gene Targeting Vector and Recombination Screening

小鼠MPI基因的打靶载体的构建和筛选

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Producing Mammary Gland Bioreactor Using Gene Targeting

基因打靶方法制备乳腺生物反应器

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Genome Editing is a a new technology to transform biological genome based on gene targeting modified, through Precise gene editing in the genomic level, we can achieve the purpose of gene knockout and knockin structural variation.

基因组编辑是建立在基因靶向修饰的基础上，对生物基因组进行改造的一项新技术，通过在基因组水平上进行精确的基因编辑，实现基因的敲除、敲入、结构变异。

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Construction of the gene targeting vector of ERP gene in BCG

卡介苗ERP基因置换型打靶载体的构建

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Homologous Recombination and Gene Targeting in Vertebrate Cells

影响动物细胞同源重组发生与基因打靶效率的分子机制

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However the techniques of multiple locus gene targeting could be an effective cover the above problems.

基因打靶的方法可以解决上述问题,但是基因打靶存在打靶效率太低的问题.

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Construction of Transgenic Silkworm Gene Targeting Vectors Based on Fibroin Light Chain

用丝心蛋白轻链基因构建转基因家蚕基因打靶载体

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With the combination of gene targeting, animal transgenic and animal cloning technologies, there have been a series of theoretical and applied research of animal genomes modified, such as fixed-point gene repair, the treatment of genetic defects and inactivation of animal unfavorable gene.

利用动物转基因技术和动物克隆技术，再结合基因打靶技术，人们开展了基因的定点修复、遗传缺陷的治疗、动物不利基因的敲除失活等一系列的动物基因组修饰的理论和应用研究。

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